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Registro Completo |
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
30/07/2020 |
Data da última atualização: |
25/08/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
IWANICKI, N. S.; MASCARIN, G. M.; MORENO, S. G.; EILENBERG, J.; DELALIBERA JUNIOR, I. |
Afiliação: |
NATASHA SANT'ANNA IWANICKI, ESALQ-USP; GABRIEL MOURA MASCARIN, CNPMA; SARA GIRO MORENO, ESALQ-USP; JORGEN EILENBERG, University of Copenhagen; ITALO DELALIBERA JUNIOR, ESALQ-USP. |
Título: |
Growth kinetic and nitrogen source optimization for liquid culture fermentation of Metarhizium robertsii blastospores and bioefficacy against the corn leafhopper Dalbulus maidis. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
World Journal of Microbiology and Biotechnology, v. 36, article 71, 2020. |
ISSN: |
1573-0972 |
DOI: |
https://doi.org/10.1007/s11274-020-02844-z |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The cosmopolitan entomopathogenic and root endophytic fungus Metarhizium robertsii has a versatile lifestyle and during liquid fermentation undergoes a dimorphic transformation from hyphae to conidia or microsclerotia, or from hyphae to blastospores. In all cases, these processes are mediated by environmental and nutritional cues. Blastospores could be used in spray applications to control arthropod pests above ground and may serve as an attractive alternative to the traditional solid-grown aerial conidial spores of Metarhizium spp. found in commercial products. Nitrogen is a vital nutrient in cell metabolism and growth; however, it is the expensive component in liquid cultures of entomopathogenic fungi. Our goals in this study were to optimize nitrogen sources and titers for maximum production of M. robertsii blastospores cultured in shake flasks at highly aerated conditions and to further determine their virulence against the corn leafhopper Dalbulus maidis, an important vector of serious pathogens in maize crops worldwide. Our fermentation studies revealed that the low-cost corn steep liquor (CSL) was the most suitable nitrogen source to improve blastospore growth in M. robertsii.The growth kinetic assays determined the optimal titer of 80 g L-1 and a yield up to 4.7 × 100.000.000 cells mL-1 within 5 days of cultivation (3 days preculture and 2 days culture), at a total cost of US$0.30 L-1. Moreover, the blastospore growth kinetic was strongly dependent on glucose and nitrogen consumptions accompanied by a slight drop in the culture pH. Insect bioassays evidenced a high virulence of these blastospores, either as dried or fresh cells, to D. maidis adults fed on maize plants. Our findings provide insights into the nutritional requirements for optimal and cost-efficient production of M. robertsii blastospores and elucidate the potential of blastospores as an ecofriendly tool against the corn leafhopper. MenosAbstract: The cosmopolitan entomopathogenic and root endophytic fungus Metarhizium robertsii has a versatile lifestyle and during liquid fermentation undergoes a dimorphic transformation from hyphae to conidia or microsclerotia, or from hyphae to blastospores. In all cases, these processes are mediated by environmental and nutritional cues. Blastospores could be used in spray applications to control arthropod pests above ground and may serve as an attractive alternative to the traditional solid-grown aerial conidial spores of Metarhizium spp. found in commercial products. Nitrogen is a vital nutrient in cell metabolism and growth; however, it is the expensive component in liquid cultures of entomopathogenic fungi. Our goals in this study were to optimize nitrogen sources and titers for maximum production of M. robertsii blastospores cultured in shake flasks at highly aerated conditions and to further determine their virulence against the corn leafhopper Dalbulus maidis, an important vector of serious pathogens in maize crops worldwide. Our fermentation studies revealed that the low-cost corn steep liquor (CSL) was the most suitable nitrogen source to improve blastospore growth in M. robertsii.The growth kinetic assays determined the optimal titer of 80 g L-1 and a yield up to 4.7 × 100.000.000 cells mL-1 within 5 days of cultivation (3 days preculture and 2 days culture), at a total cost of US$0.30 L-1. Moreover, the blastospore growth kinetic was strongly dependent on gluco... Mostrar Tudo |
Palavras-Chave: |
Biocontrol; Dimorphic growth; Fungal entomopathogen; Fungal nutrition. |
Thesagro: |
Fungo Para Controle Biológico; Milho; Praga de Planta; Zea Mays. |
Thesaurus Nal: |
Biological control agents; Corn; Entomopathogenic fungi; Metarhizium robertsii; Plant pests; Virulence. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 03118naa a2200361 a 4500 001 2124093 005 2021-08-25 008 2020 bl uuuu u00u1 u #d 022 $a1573-0972 024 7 $ahttps://doi.org/10.1007/s11274-020-02844-z$2DOI 100 1 $aIWANICKI, N. S. 245 $aGrowth kinetic and nitrogen source optimization for liquid culture fermentation of Metarhizium robertsii blastospores and bioefficacy against the corn leafhopper Dalbulus maidis.$h[electronic resource] 260 $c2020 520 $aAbstract: The cosmopolitan entomopathogenic and root endophytic fungus Metarhizium robertsii has a versatile lifestyle and during liquid fermentation undergoes a dimorphic transformation from hyphae to conidia or microsclerotia, or from hyphae to blastospores. In all cases, these processes are mediated by environmental and nutritional cues. Blastospores could be used in spray applications to control arthropod pests above ground and may serve as an attractive alternative to the traditional solid-grown aerial conidial spores of Metarhizium spp. found in commercial products. Nitrogen is a vital nutrient in cell metabolism and growth; however, it is the expensive component in liquid cultures of entomopathogenic fungi. Our goals in this study were to optimize nitrogen sources and titers for maximum production of M. robertsii blastospores cultured in shake flasks at highly aerated conditions and to further determine their virulence against the corn leafhopper Dalbulus maidis, an important vector of serious pathogens in maize crops worldwide. Our fermentation studies revealed that the low-cost corn steep liquor (CSL) was the most suitable nitrogen source to improve blastospore growth in M. robertsii.The growth kinetic assays determined the optimal titer of 80 g L-1 and a yield up to 4.7 × 100.000.000 cells mL-1 within 5 days of cultivation (3 days preculture and 2 days culture), at a total cost of US$0.30 L-1. Moreover, the blastospore growth kinetic was strongly dependent on glucose and nitrogen consumptions accompanied by a slight drop in the culture pH. Insect bioassays evidenced a high virulence of these blastospores, either as dried or fresh cells, to D. maidis adults fed on maize plants. Our findings provide insights into the nutritional requirements for optimal and cost-efficient production of M. robertsii blastospores and elucidate the potential of blastospores as an ecofriendly tool against the corn leafhopper. 650 $aBiological control agents 650 $aCorn 650 $aEntomopathogenic fungi 650 $aMetarhizium robertsii 650 $aPlant pests 650 $aVirulence 650 $aFungo Para Controle Biológico 650 $aMilho 650 $aPraga de Planta 650 $aZea Mays 653 $aBiocontrol 653 $aDimorphic growth 653 $aFungal entomopathogen 653 $aFungal nutrition 700 1 $aMASCARIN, G. M. 700 1 $aMORENO, S. G. 700 1 $aEILENBERG, J. 700 1 $aDELALIBERA JUNIOR, I. 773 $tWorld Journal of Microbiology and Biotechnology$gv. 36, article 71, 2020.
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Embrapa Meio Ambiente (CNPMA) |
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Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
22/10/1997 |
Data da última atualização: |
01/06/2006 |
Autoria: |
MIRANDA, L. C. |
Título: |
Secagem de sementes de soja em silo com distribuicao radial do fluxo de ar. |
Ano de publicação: |
1997 |
Fonte/Imprenta: |
Piracicaba: ESALQ, 1997. |
Páginas: |
76p. |
Idioma: |
Português |
Notas: |
Tese Doutorado. |
Conteúdo: |
A secagem de sementes de soja (Glycine max (L.) Merrill) foi estudada, quanto as consequencias das variacoes no fluxo e na temperatura do ar insuflado considerando o periodo de secagem e a posicao das sementes na massa, utilizando um secador estacionario de distribuicao radial do fluxo de ar. Na pesquisa, foram caracterizados o ar ambiente, o ar insuflado, as temperaturas e os teores de agua da massa, as equacoes de secagem e a qualidade fisiologica (germinacao e vigor) das sementes durante o armazenamento. Admitindo os intervalos de 26,9 a 33,2 m3/min/t, para o fluxo, e de 42 a 50C, para a temperatura do ar insuflado radialmente durante a secagem das sementes de soja em silos, conclui-se que a elevacao na temperatura demanda reducao no fluxo e, inversamente, o aumento no fluxo demanda reducao na temperatura. |
Palavras-Chave: |
Brasil; Grain; Sao Paulo; Seed; Soybean. |
Thesagro: |
Glycine Max; Grão; Secador; Secagem; Semente; Silo; Soja. |
Thesaurus NAL: |
Brazil; drying; storage. |
Categoria do assunto: |
-- F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 01525nam a2200313 a 4500 001 1459705 005 2006-06-01 008 1997 bl uuuu m 00u1 u #d 100 1 $aMIRANDA, L. C. 245 $aSecagem de sementes de soja em silo com distribuicao radial do fluxo de ar. 260 $aPiracicaba: ESALQ$c1997 300 $a76p. 500 $aTese Doutorado. 520 $aA secagem de sementes de soja (Glycine max (L.) Merrill) foi estudada, quanto as consequencias das variacoes no fluxo e na temperatura do ar insuflado considerando o periodo de secagem e a posicao das sementes na massa, utilizando um secador estacionario de distribuicao radial do fluxo de ar. Na pesquisa, foram caracterizados o ar ambiente, o ar insuflado, as temperaturas e os teores de agua da massa, as equacoes de secagem e a qualidade fisiologica (germinacao e vigor) das sementes durante o armazenamento. Admitindo os intervalos de 26,9 a 33,2 m3/min/t, para o fluxo, e de 42 a 50C, para a temperatura do ar insuflado radialmente durante a secagem das sementes de soja em silos, conclui-se que a elevacao na temperatura demanda reducao no fluxo e, inversamente, o aumento no fluxo demanda reducao na temperatura. 650 $aBrazil 650 $adrying 650 $astorage 650 $aGlycine Max 650 $aGrão 650 $aSecador 650 $aSecagem 650 $aSemente 650 $aSilo 650 $aSoja 653 $aBrasil 653 $aGrain 653 $aSao Paulo 653 $aSeed 653 $aSoybean
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